SKRINING FITOKIMIA EKSTRAK DAUN DAN RANTING GLODOKAN TIANG (Polyalthia longifolia L.)

Yunita; Nadiaro Risky Nasution; Rozalia; Musfirah  Handayani; Desy  Kurniawati; Della Rosalynna Stiadi

doi:10.36989/didaktik.v12i3.16624

Yunita Universitas Negeri Padang
Nadiaro Risky Nasution Universitas Negeri Padang
Rozalia Universitas Negeri Padang
Musfirah Handayani Universitas Negeri Padang
Desy Kurniawati Universitas Negeri Padang
Della Rosalynna Stiadi Universitas Negeri Padang

DOI: https://doi.org/10.36989/didaktik.v12i3.16624

Keywords: Maceration, Secondary metabolites, FTIR

Abstract

This study aimed to identify the secondary metabolite compounds present in leaf and twig extracts of glodokan tiang (Polyalthia longifolia L.) using phytochemical screening and Fourier Transform Infrared (FTIR) analysis. Dried, powdered samples were extracted by maceration with ethyl acetate and 96% ethanol. The extects were qualitatively screened for steroids, terpenoids, alkaloids, phenolics, flaavonoids, and saponins. FTIR analysis (4000-450 cm^-1) was used to identify functional groups. Both leaf and twig extracts contained various secondary metabolites, with 96% ethanol extracts showing a broader range of compounds than ethyl acetate extracts. Ethanol leaf extract tested positive for steroids, terpenoids, alkaloids, flavonoids, and saponins. FTIR spectra confirmed the presence of O-H, C-H, C=O, and C-O functional groups, supporting the existence of bioactive secondary metabolites. These findings indicate that glodokan tiang leaves and twigs are promising natural sources of bioactive compounds, and that 96% ethanol is a more effective solvent for extracting secondary metabolites.

References

Amin, N. (2015). Tumbuhan Peneduh di Hutan Kota Banda Aceh Sebagai Media Pembelajaran Biologi. Prosiding Seminar Nasional Biotik, 495–501.

Anam, C., Sirojudin, & Sofjan Firdausi, K. (2007). Analisis Gugus Fungsi pada Ekstrak Uji, Bensin dan Spritus Menggunakan Metode Spektroskopi FTIR. Berkala Fisika, 10(1), 79–85.

Balasundram, N., Sundram, K., & Samman, S. (2006). Phenolic compounds in plants and agri-industrial by-products: Antioxidant activity, occurrence, and potential uses. Food Chemistry, 99(1), 191–203.

Ganaim Fasya, A., Riska Dinasti, A., Syofiyah, M., Maghfiroh Rahmawati, L., Millati, N., Aulia Safitri, D., Handoko, S., Hanapi, A., & Ningsih, R. (2016). Ekstraksi, Hidrolisis dan Partisi Metabolit Sekunder dari Mikroalga Chlorella sp. ALCHEMY : Journal of Chemistry, 5(1), 5–9.

Handoyo, D. L. Y. (2020). Pengaruh Lama Waktu Maserasi (Perendaman) Terhadap Kekentalan Ekstrak Daun Sirih (Piper Betle). Jurnal Farmasi Tinctura, 2(1), 34–41.

Hanifatun Nikmah, U., Samodra, G., & Yuda Kusuma, I. (2022). Skrining Fitokimia Senyawa Metabolit Sekunder dan Uji Karakteristik Kadar Air Ekstrak Biji Mahoni (Swietenia Mahagoni. (L) Jacq). Seminar Nasional Penelitian Dan Pengebdian Kepada Masyarakat (SNPPKM), 120–125.

Harahap, S. N., & Situmorang, N. (2021). Skrining Fitokimia dari Senyawa Metabolit Sekunder Buah Jambu Biji Merah (Psidium guajava L.). EduMatSains: Jurnal Pendidikan, Matematika Dan Sains, 5(2), 153–164.

Harbone, J. B. (1987). Metode Fitokimia: Penuntun Cara Modern Menganalisis Tumbuhan. Terbitan Kedua. Bandung: ITB.

Hasibuan, A. S., & Edrianto, V. (2021). Sosialisasi Skrining Ekstrak Fitokimia Ekstrak Etanol Umbi Bawang Merah (Allium cepa L.). JURNAL PENGMAS KESTRA (JPK), 1(1), 80–84.

Jafar, W., Masriany, & Sukmawaty, E. (2020). Uji Fitokimia Ekstrak Etanol Bunga Pohon Hujan (Spathodea campanulata) Secara In Vitro. Prosiding Seminar Nasional Biotik, 328–334.

Munhoz, V. M., Longhini, R., Souza, J. R. P., Zequi, J. A. C., Mello, E. V. S. L., Lopes, G. C., & Mello, J. C. P. (2014). Extraction of flavonoids from Tagetes patula: Process optimization and screening for biological activity. Revista Brasileira de Farmacognosia, 24(5), 576–583.

Muyonga, J. H., Cole, C. G. B., Duodu, K. G. (2004). Fourier transform infrared (FTIR) spectroscopic study of acid soluble collagen and gelatin from skins and bones of young and adult Nile perch (Lates niloticus). Food Chemistry.

Nurfirzatulloh, I., Suherti, I., Insani, M., Shafira, R. A., Abriyani, E. (2023). Literature Review Article: Identifikasi Gugus Fungsi Tanin Pada Beberapa Tumbuhan Dengan Instrumen FTIR. Jurnal Ilmiah Wahana Pendidikan, 9(4), 201-209.

Oktavia, F. D., & Sutoyo, S. (2021). Skrining Fitokimia, Kandungan Flavonoid total, Dan Aktivitas Antioksidan Ekstrak Etanol Tumbuhan Selaginella doederleinii. Jurnal Kimia Riset, 6(2), 141–153.

Shofi, Muh., & Munawaroh, S. (2023). Uji Aktivitas Sel Kanker Payudara T47D Ekstrak Etil Asetat Daun Glodokan Tiang (Polyalthia longifolia L.) Secara In Vitro. Bioscientist: Jurnal Ilmiah Biologi, 11(2), 1493–1500.

Singh, J. P., Kaur, A., Shevkani, K., & Singh, N. (2015). Influence of jambolan (Syzygium cumini) and xanthan gum incorporation on the physicochemical, antioxidant and sensory properties of gluten-free eggless rice muffins. International Journal of Food Science and Technology, 50(5), 1–8.

Sulistyarini, I., Sari, A. D., & Wicaksono, A. T. (2020). Skrining Fitokimia Senyawa Metabolit Sekunder Batang Buah Naga (Hylocereus polyrhizus). Jurnal Ilmiah Cendekia Eksakta, 5(1), 56–62.

Vita Wendersteyt, N., Wewengkang, D. S., & Sumantri Abdullah, S. (2021). Uji Aktivitas Antimikroba Dari Ekstrak Dan Fraksi Ascidian Herdmania momus Dari Perairan Pulau Bangka Likupang Terhadap Pertumbuhan Mikroba Staphylococcus aureus, Salmonella typhimurium Dan Candida albicans. Pharmacon, 10(1), 706–712.

Warni, J., Marliah, A., & Erida, G. (2022). Uji Aktivitas Bioherbisida Ekstrak Etil Asetat Teki (Cyperus rotundus L.) Terhadap Pertumbuhan Gulma Bayam Duri (Amaranthus spinosus L.). Jurnal Ilmiah Mahasiswa Pertanian, 7(2), 47–54.

Wulandari, L. (2011). Kromatografi Lapis Tipis (1st ed.). PT. Taman Kampus Presindo.